MATLAB PROJECT
Extending the hybrid
surgical guidance concept with freehand fluorescence tomography
Abstract:
Within image-guided
surgery, ‘hybrid’ guidance technologies have been used to integrate the
complementary features of radioactive guidance and fluorescence guidance. Here,
we explore how the generation of a novel freehand fluorescence (fhFluo) imaging
approach complements freehand SPECT (fhSPECT) in a hybrid setup. Near-infrared
optical tracking was used to register the position and orientation of a hybrid
opto-nuclear detection probe, while recording its readings. Dedicated look-up
table models were used for 3D reconstruction. In phantom and excised tissue
settings (i.e. flat-surface human skin explants), fhSPECT and fhFluo were
investigated for image resolution and in-tissue signal penetration. Finally,
the combined potential of these freehand technologies was evaluated on prostate
and lymph node specimens of prostate cancer patients receiving prostatectomy
and sentinel lymph node dissection (tracers: indocyanine green (ICG) +
99mTc-nanocolloid or ICG-99mTc-nanocolloid). After hardware and software
integration, the hybrid setup created 3D nuclear and fluorescence tomography
scans. Imaging resolution of fhFluo (1 mm) was superior to that of fhSPECT (6
mm). Fluorescence modalities were confined to a maximum depth of 0.5 cm, while
nuclear modalities were usable at all evaluated depths (<2 cm). Both fhSPECT
and fhFluo enabled augmented-and virtual-reality navigation towards segmented
image hotspots, including relative hotspot quantification with an accuracy of
3.9 and 4.1%, respectively. Imaging in surgical specimens confirmed these
trends (fhSPECT: in-depth detectability, low resolution; fhFluo: superior
resolution, superficial detectability). Overall, when radioactive and
fluorescent tracer signatures are used, fhFluo has complementary value to fhSPECT.
Combined the freehand technologies render a unique hybrid imaging and
navigation modality.
No comments:
Post a Comment